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KMID : 0848120130380020043
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International Journal of Oral Biology
2013 Volume.38 No. 2 p.43 ~ p.49
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Development of Streptococcus sanguinis-, Streptococcus parasanguinis-, and Streptococcus gordonii-PCR Primers Based on the Nucleotide Sequences of Species-specific DNA Probes Screened by Inverted Dot Blot Hybridization
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Kook Joong-Ki
Park Soon-Nang
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Abstract
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The objective of this study was to develop PCR primersthat are specific for Streptococcus sanguinis, Streptococcusparasanguinis, and Streptococcus gordonii. We designedthe S. sanguinis-, S. parasanguinis-, and S. gordoniispecificprimers, Ssa21-F3/Ssa21-R2, Spa17-F/Spa17-R, andSgo41-F1/Sgo41-R1 respectively, based on the nucleotidesequences of the Ssa21, Spa17, and Sgo41 DNA probesthat were screened using inverted dot blot hybridization(IDBH). The species-specificity of these primers was assessedagainst 43 strains of mitis group streptococci, includingclinical strains of S. sanguinis, S. parasanguinis,and S. gordonii. The resulting PCR data revealed thatspecies-specific amplicons had been obtained from allstrains of the target species tested, and that none of these ampliconsoccurred in any other strains from other species.These results suggest that the Ssa21-F3/Ssa21-R2, Spa17-F/Spa17-R, and Sgo41-F1/Sgo41-R1 primers may be usefulin detecting S. sanguinis, S. parasanguinis, and S. gordoniiat the species level, respectively.
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KEYWORD
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Streptococcus gordonii, Streptococcus sanguinis, Streptococcus parasanguinis, species-specific PCR primer, IDBH
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